About   Help   FAQ
Transgene Detail
Symbol: Tg(Tnfrsf19-cre/ERT2)1Kori
Name: transgene insertion 1, Vladimir Korinek
MGI ID: MGI:5575757
Synonyms: TROY-CreERT2
Transgene: Tg(Tnfrsf19-cre/ERT2)1Kori  Location: unknown  
Alliance: Tg(Tnfrsf19-cre/ERT2)1Kori page
Strain of Origin:  C57BL/6NCrl
Transgene Type:    Transgenic (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
Tg(Tnfrsf19-cre/ERT2)1Kori expression driven by 1 gene
Mutation detailsThe transgene was derived from the mouse bacterial artificial chromosome (BAC) RP23-166C22, which contains the entire tumor necrosis factor receptor superfamily 19 (Tnfrsf19; also called Troy) gene with 65 kb of 5' and 88 kb of 3' flanking genomic DNA. A cDNA encoding a fusion protein comprising cre recombinase joined to a modified human estrogen receptor (creERT2), followed by the rabbit beta-globin polyadenylation signal, was inserted in-frame at the translation initiation codon in the second exon; the timing and location of creERT2 expression are determined by Tnfrsf19 regulatory sequences, but it remains inactive until bound to tamoxifen and transported to the nucleus. X-Gal staining and in-situ hybridization with a Tnfrsf19 anti-sense RNA of E14 embryos bearing this transgene and the Gt(ROSA)26Sortm1Sor cre reporter collected one day after their dams' treatment with tamoxifen showed coordinate cre recombinase-induced lacZ and endogenous Tnfrsf19 mRNA expression, notably in the telencephalon, cerebral plate, ear, maxilla, vibrissae, dorsal root ganglia, and mammary gland primordia. Three transgenic founder lines were obtained, all exhibiting virtually identical Cre recombinase activity patterns. (J:211697)
 Tissue activity of this recombinase allele
Driver: Tnfrsf19 (mouse)
Summary of all recombinase alleles driven by Tnfrsf19.

View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:211697 Fafilek B, et al., Troy, a tumor necrosis factor receptor family member, interacts with lgr5 to inhibit wnt signaling in intestinal stem cells. Gastroenterology. 2013 Feb;144(2):381-91
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
MGI 6.24
The Jackson Laboratory