Tg(Tnfrsf19-cre/ERT2)1Kori
Transgene Detail

Summary

• Symbol: Tg(Tnfrsf19-cre/ERT2)1Kori
• Name: transgene insertion 1, Vladimir Korinek
• MGI ID: MGI:5575757
• Synonyms: TROY-CreERT2
• Transgene: Tg(Tnfrsf19-cre/ERT2)1Kori Location: unknown
• Alliance: Tg(Tnfrsf19-cre/ERT2)1Kori page

Transgene origin

• Strain of Origin: C57BL/6NCrl

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Tnfrsf19-cre/ERT2)1Kori expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tnfrsf19 (MGI:1352474)

• Mutation details: The transgene was derived from the mouse bacterial artificial chromosome (BAC) RP23-166C22, which contains the entire tumor necrosis factor receptor superfamily 19 (Tnfrsf19; also called Troy) gene with 65 kb of 5' and 88 kb of 3' flanking genomic DNA. A cDNA encoding a fusion protein comprising cre recombinase joined to a modified human estrogen receptor (creERT2), followed by the rabbit beta-globin polyadenylation signal, was inserted in-frame at the translation initiation codon in the second exon; the timing and location of creERT2 expression are determined by Tnfrsf19 regulatory sequences, but it remains inactive until bound to tamoxifen and transported to the nucleus. X-Gal staining and in-situ hybridization with a Tnfrsf19 anti-sense RNA of E14 embryos bearing this transgene and the Gt(ROSA)26Sor^tm1Sor cre reporter collected one day after their dams' treatment with tamoxifen showed coordinate cre recombinase-induced lacZ and endogenous Tnfrsf19 mRNA expression, notably in the telencephalon, cerebral plate, ear, maxilla, vibrissae, dorsal root ganglia, and mammary gland primordia. Three transgenic founder lines were obtained, all exhibiting virtually identical Cre recombinase activity patterns. (J:211697)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tnfrsf19 (mouse) Summary of all recombinase alleles driven by Tnfrsf19. Alliance mouse Tnfrsf19 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:211697 Fafilek B, et al., Troy, a tumor necrosis factor receptor family member, interacts with lgr5 to inhibit wnt signaling in intestinal stem cells. Gastroenterology. 2013 Feb;144(2):381-91
• All: 3 reference(s)