Tg(Tg-cre/ERT2)1Kero
Transgene Detail

Summary

• Symbol: Tg(Tg-cre/ERT2)1Kero
• Name: transgene insertion 1, Jukka Kero
• MGI ID: MGI:5575412
• Synonyms: TgCreER^T2
• Transgene: Tg(Tg-cre/ERT2)1Kero Location: unknown
• Alliance: Tg(Tg-cre/ERT2)1Kero page

Transgene origin

• Strain of Origin: C57BL/6NCrl

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Tg-cre/ERT2)1Kero expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Tg

• Mutation details: RedE/RedT-recombination was used to insert a cassette encoding tamoxifen-inducible cre (cre/ERT2), an frt-flanked ampicillin selection marker, and the bovine growth hormone polyadenylation signal at the ATG start codon of the thyroglobulin (Tg) gene in a P1 artificial chromosome (PAC). Following recombination, flp-mediated deletion of the ampicillin marker was performed. The verified construct was linearized, then used for injection of C57BL/6 fertilized oocytes. 5/35 pups tested positive for cre by PCR. Founders were tested for specificity and inducibility of cre recombinase by breeding to lacZ reporter mice and treating double mutants with tamoxifen. 4 founders showed cre activity only in the thyroid gland, and one line with strong and homogenous cre activity was chosen for detailed analyses. (J:211398)

Generation of Tg(Tg-cre/ERT2)1Kero.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tg (species not specified) Summary of all recombinase alleles driven by Tg. Comparative matrix view of recombinase activities

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:211398 Undeutsch H, et al., A mouse model with tamoxifen-inducible thyrocyte-specific cre recombinase activity. Genesis. 2014 Apr;52(4):333-40
• All: 6 reference(s)