Gt(ROSA)26Sor^{tm1(CAG-ECFP/EYFP*)Phep}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-ECFP/EYFP*)Phep}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Paul A Heppenstall
• MGI ID: MGI:5573198
• Synonyms: Gt(ROSA)26Sor^tm1Phep, Rosa26::Cl-Sensor
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-ECFP/EYFP*)Phep} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209636
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The inserted sequence encodes a cre recombinase-inducible, sensitive, non-invasive fluorescent reporter of intracellular chloride ion concentration [Cl^-] (Kapp ~30 mM) and pH ((pKalpha 7.1-8.0 pH units at different [Cl^-]) under physiologic conditions. This reporter molecule, which the authors call Cl-Sensor, takes advantage of the quenching of EYFP by Cl^- and permits fluorescence resonance energy transfer- (FRET-) based ratiometric measurement of [Cl^-] in living cells. The chimeric reporter protein comprises enhanced cyan fluorescent protein (ECFP) joined by a 20-amino acid linker to enhanced yellow fluorescent protein (EYFP) containing three amino acid substitutions (His148Gln, Ile152Leu and Val163Ser) that increase its [Cl^-] sensitivity five-fold. The targeting construct contained the CAG promoter (consisting of the cytomegalovirus immediate early (CMV-IE) enhancer and the chicken beta-actin promoter); the adenovirus splice donor and splice acceptor sites; a neomycin resistance (neo^r) gene flanked 5' by an inverted loxP site and a Kozak consensus sequence and 3' by two copies of the polyadenylation site from the bovine growth hormone gene and a mutant loxP2272 site; the Cl-Sensor cDNA followed by the SV40 polyadentylation site, in reverse transcriptional orientation relative to neo^r; a loxP site and an inverted mutant loxP2272 site. This construct was inserted into the original Gt(ROSA)26Sor targeting vector at the unique XbaI site approximately 300 bp 5' of the original gene trap integration site. (J:209636, J:211025)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:209636 Batti L, et al., Transgenic mouse lines for non-invasive ratiometric monitoring of intracellular chloride. Front Mol Neurosci. 2013;6:11
• All: 4 reference(s)