Gt(ROSA)26Sor^tm1(uprt)Tnr
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1(uprt)Tnr
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Martin Turner
• MGI ID: MGI:5573190
• Synonyms: Gt(Rosa)26Sor^tm1(UPRT)Tnr
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm1(uprt)Tnr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^tm1(uprt)Tnr expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  T. gondii	TGME49_112480 (7900442)

• Mutation details: A codon-optimized cDNA encoding the salvage enzyme uracil phosphoribosyltransferase (UPRT) from the protozoan parasite Toxoplasma gondii is expressed under the control of the Gt(ROSA)26Sor promoter upon activation by cre recombinase. In cells expressing cre recombinase, and thus T gondii UPRT, 4-thiouracil injected into the mouse is converted to 4-thiouridine that is then incorporated into mRNA; this facilitates isolation and characterization of the transcriptome of a specifc cell population. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)