Tg(CMV-EYFP/DsRed2)1Mklv
Transgene Detail

Summary

• Symbol: Tg(CMV-EYFP/DsRed2)1Mklv
• Name: transgene insertion 1, Andrey Mikhailov
• MGI ID: MGI:5573173
• Synonyms: EYFP-CCS-DsRed, EYFP-DEVD-DsRed FRET, FRET-casp3, TandoMice, Yellow Fluorescent Protein(EYFP)-caspase-3 cleavage site(CCS)-Red Fluorescent Protein (DsRed)
• Transgene: Tg(CMV-EYFP/DsRed2)1Mklv Location: unknown
• Alliance: Tg(CMV-EYFP/DsRed2)1Mklv page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The cytomegalovirus enhancer/promoter drives expression of a chimeric protein in which enhanced yellow fluorescent protein (EYFP) is separated from Discosoma red fluorescent protein 2 (DsRed2) by a 19-amino acid peptide linker that includes the capase-3 cleavage site (Asp-Glu-Val-Asp). When cells expressing the intact chimeric protein are excited with a 450-490 nm light or with a 488 nm argon laser, they strongly fluoresce red due to fluorescence resonance energy transfer (FRET) from donor EYFP to acceptor DsRed2. Upon caspase 3 activation, cleavage of the joining peptide chain results in increased EYFP fluorescence and concomitant decreased DsRed fluorescence, which is correlated with increased pro-caspase 3 processing. This serves as a sensitive reporter of caspase 3 activation upon induction of apoptosis. (J:82809, J:209202)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 2 reference(s)