Cldn3^tm1.1Reb
Targeted Allele Detail

Summary

• Symbol: Cldn3^tm1.1Reb
• Name: claudin 3; targeted mutation 1.1, Robert E Braun
• MGI ID: MGI:5571706
• Synonyms: Cldn3^-
• Gene: Cldn3 Location: Chr5:135015068-135016326 bp, + strand Genetic Position: Chr5, 74.93 cM
• Alliance: Cldn3^tm1.1Reb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:210656
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S1/SvImJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to insert a single loxP site upstream of exon 1 and a frt-flanked neomycin resistance cassette followed by another loxP site downstream of exon 1. Cre-mediated recombination removed the floxed neo cassette and exon 1. Western blot analysis confirmed the absence of protein in the testes. Analysis by RT-qPCR confirmed the absence of transcript in the testes, liver and lung. (J:210656)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cldn3 Mutation: 12 strains or lines available

References

• Original: J:210656 Chakraborty P, et al., Androgen-dependent sertoli cell tight junction remodeling is mediated by multiple tight junction components. Mol Endocrinol. 2014 Jul;28(7):1055-72
• All: 1 reference(s)