Lmna^tm9Lgf
Targeted Allele Detail

Summary

• Symbol: Lmna^tm9Lgf
• Name: lamin A; targeted mutation 9, Loren G Fong
• MGI ID: MGI:5571496
• Synonyms: Lmna^PLAO-5NT
• Gene: Lmna Location: Chr3:88388455-88413842 bp, - strand Genetic Position: Chr3, 38.84 cM
• Alliance: Lmna^tm9Lgf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207144
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion, Nucleotide substitutions
• Mutation details: Introns 10 and 11 of Lmna were deleted thereby abolishing production of lamin C and producing only prelamin A. Exon 12 was modified such that the Mir9 seed-binding sequence in prelamin A's 3' UTR (CCAAAG) was modified to ACCCTG. A floxed neomycin resistance cassette was inserted downstream of the modified exon 12. Western blot analysis confirmed the increased expression of lamin A protein in cerebral cortex and the cerebellum. (J:207144)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lmna Mutation: 82 strains or lines available

References

• Original: J:207144 Jung HJ, et al., New Lmna knock-in mice provide a molecular mechanism for the 'segmental aging' in Hutchinson-Gilford progeria syndrome. Hum Mol Genet. 2014 Mar 15;23(6):1506-15
• All: 1 reference(s)