Tg(Prnp*S170N*N174T)1020Aag
Transgene Detail

Summary

• Symbol: Tg(Prnp*S170N*N174T)1020Aag
• Name: transgene insertion 1020, Adriano Aguzzi
• MGI ID: MGI:5569540
• Synonyms: RL-PrP, tg1020
• Transgene: Tg(Prnp*S170N*N174T)1020Aag Location: unknown
• Alliance: Tg(Prnp*S170N*N174T)1020Aag page

Tg(Prnp*S170N*N174T)1020Aag/0 Prnp^tm1Cwe/Prnp^tm1Cwe mice develop a lethal neurologic disease with lesions in the brain and muscle

Show the 3 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: B6;129S7-Prnp^tm1Cwe

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp*S170N*N174T)1020Aag expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Prnp (MGI:97769)	Transgene codes for a protein with serine to asparagine and asparagine to threonine mutations.

• Mutation details: This transgene consists of a murine Prnp mini-gene with two point mutations, G to A and A to C, at nucleotide positions 506 and 518, that affect the structure of the globular domain. This results in a mouse-elk Prnp chimeric molecule termed rigid loop Prnp. This line has approximately 45 copies of the transgene and expresses about 2- to 3-fold of wild-type levels in the brain. (J:143528)

The plasmid used for the generation of the Tg(Prnp*S170N*N174T)1020Aag transgenic mouse

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:143528 Sigurdson CJ, et al., De novo generation of a transmissible spongiform encephalopathy by mouse transgenesis. Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):304-9
• All: 2 reference(s)