Setd1b^tm1.3Afst
Targeted Allele Detail

Summary

• Symbol: Setd1b^tm1.3Afst
• Name: SET domain containing 1B; targeted mutation 1.3, A Francis Stewart
• MGI ID: MGI:5568945
• Synonyms: Setd1b^FDC
• Gene: Setd1b Location: Chr5:123280256-123306692 bp, + strand Genetic Position: Chr5, 62.8 cM
• Alliance: Setd1b^tm1.3Afst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:208420
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: An FRT-flanked stop cassette containing a lacZ reporter, followed by loxP-flanked exon 5 and rox-flanked PGK-hygromycin cassette were inserted. Dre-recombination removed the PGK-hygromycin selection cassette. Flp mediated recombination removed the stop cassette leaving exon 5 floxed. Cre mediated recombination excised exon 5, resulting in frameshift. Western analysis confirmed lost of Setd1b protein. (J:208420)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Setd1b Mutation: 49 strains or lines available

References

• Original: J:208420 Bledau AS, et al., The H3K4 methyltransferase Setd1a is first required at the epiblast stage, whereas Setd1b becomes essential after gastrulation. Development. 2014 Mar;141(5):1022-35
• All: 2 reference(s)