Rgs8^tm1.1Atai
Targeted Allele Detail

Summary

• Symbol: Rgs8^tm1.1Atai
• Name: regulator of G-protein signaling 8; targeted mutation 1.1, Atsu Aiba
• MGI ID: MGI:5566702
• Gene: Rgs8 Location: Chr1:153528612-153573415 bp, + strand Genetic Position: Chr1, 65.41 cM, cytoband G2
• Alliance: Rgs8^tm1.1Atai page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:208753
• Parent Cell Line: EB3 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin resistance cassette was inserted upstream of exon 4. An additional loxP site was inserted downstream of exon 5. Cre-mediated recombination removed the selection cassette, exon 4 and exon 5. Western blot analysis confirmed the absence of protein expression in the cerebellum. (J:208753)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rgs8 Mutation: 17 strains or lines available

Notes

ES cell line = EB3, a mutant E14TG2a line (129P2/OlaHsd). Targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette inactivated the Pou5f1 allele. J:208753 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.

References

• Original: J:208753 Kuwata H, et al., Generation of RGS8 null mutant mice by Cre/loxP system. Kobe J Med Sci. 2007;53(6):275-81
• All: 1 reference(s)