Tg(Myh6-Kcne1)#Ven
Transgene Detail

Summary

• Symbol: Tg(Myh6-Kcne1)#Ven
• Name: transgene insertion, Bjorn Vennstrom
• MGI ID: MGI:5566532
• Synonyms: alphaMyHC-KCNE1, alphaMyHC P-KCNE1 transgene, Tg(MYH-Kcne1)Ven
• Transgene: Tg(Myh6-Kcne1)#Ven Location: unknown
• Alliance: Tg(Myh6-Kcne1)#Ven page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-Kcne1)#Ven expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Kcne1 (MGI:96673)

• Mutation details: The transgene contains the cardiac-specific mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter through the translation initiation codon, in exon 3, joined to the coding sequence for mouse potassium voltage-gated channel, Isk-related subfamily, member 1 (Kcne1; GenBank: X60457 nt 1-611), followed by the human growth hormone polyadenylation signal. Northern blot analysis reveals markedly elevated levels of Kcne1 mRNA in heart, but not in kidney, of transgenic mice. Western blot analysis using an antibody to a C-terminal epitope of KCNE1 yields three bands representing native and glycosylated forms of the protein in extracts of hearts from transgenic, but not from wild-type, mice. (J:30467, J:175595)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Of five transgenic founders, two were used to generate transgenic lines of mice. Data are not associated with individual lines. (J:175595)

References

• Original: J:175595 Mansen A, et al., Thyroid hormone receptor alpha can control action potential duration in mouse ventricular myocytes through the KCNE1 ion channel subunit. Acta Physiol (Oxf). 2010 Feb;198(2):133-42
• All: 2 reference(s)