Tg(Prnp)E1Rchi
Transgene Detail

Summary

• Symbol: Tg(Prnp)E1Rchi
• Name: transgene insertion E1, Roberto Chiesa
• MGI ID: MGI:5563239
• Synonyms: Tg(WT-E1)
• Transgene: Tg(Prnp)E1Rchi Location: unknown
• Alliance: Tg(Prnp)E1Rchi page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA/J)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp)E1Rchi expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Prnp (MGI:97769)

• Mutation details: The transgene consists of 12 kb of the mouse Prnp promoter region, which includes intron 1, joined to a wild-type mouse Prnp cDNA. The transgene also includes an epitope tag for monoclonal antibody 3F4. Hemizygous mice of line E1 carry 9 copies of the transgene construct, as determined by quantitative Southern blot analysis. Western blot analysis of brain homogenates using the 3F4 antibody demonstrates expression of the mutant prion protein at 1.8-fold the level of endogenous hamster PrP. (J:51891)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:51891 Chiesa R, et al., Neurological illness in transgenic mice expressing a prion protein with an insertional mutation. Neuron. 1998 Dec;21(6):1339-51
• All: 9 reference(s)