Gt(ROSA)26Sor^{tm1(CAG-EGFP,-tDsRed)Utr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-EGFP,-tDsRed)Utr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, University of Tsukuba Laboratory Animal Resource Center
• MGI ID: MGI:5560773
• Synonyms: R26GRR, ROSA26^{CAG-EGFP/tdsRed}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-EGFP,-tDsRed)Utr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205139
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6NCrlCrlj

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The cassette used to generate the targeting vector contained the CAG promoter, the EGFP sequence with loxP sites flanking the coding region, a rabbit polyA signal, and a FLAG-tagged tandem version of the DsRed fluorophore (tDsRed) with the SV40 polyA signal. This construct, ligated to homology arms and an Frt-flanked PGK-neo selection marker, was linearized and electroporated into ES cells for homologous recombination with the Gt(ROSA)26Sor locus. (J:205139)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

Notes

ES cell line = B6N-22

References

• Original: J:205139 Hasegawa Y, et al., Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination. Exp Anim. 2013;62(4):295-304
• All: 20 reference(s)