Gt(ROSA)26Sor^{tm1.1(Phc2*,EGFP)Hko}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(Phc2*,EGFP)Hko}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Haruhiko Koseki
• MGI ID: MGI:5559321
• Synonyms: FM2(+)
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(Phc2*,EGFP)Hko} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204987
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(Phc2*,EGFP)Hko} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Phc2 (MGI:1860454)

• Mutation details: The targeting construct introduced into the locus a floxed neomycin resistance/STOP cassette, a FLAG-tagged modified Phc cDNA with the L307R mutation (FM2), and IRES-EGFP. Cre-mediated recombination removed the selection/STOP cassette to allow for expression of the cDNA and reporter gene. (J:204987)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:204987 Isono K, et al., SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing. Dev Cell. 2013 Sep 30;26(6):565-77
• All: 1 reference(s)