Ntf3^tm1.1Eafx
Targeted Allele Detail

Summary

• Symbol: Ntf3^tm1.1Eafx
• Name: neurotrophin 3; targeted mutation 1.1, Edward A Fox
• MGI ID: MGI:5559188
• Synonyms: NT-3^lox-lacZ
• Gene: Ntf3 Location: Chr6:126078375-126143703 bp, - strand Genetic Position: Chr6, 60.45 cM
• Alliance: Ntf3^tm1.1Eafx page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201812
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Reporter)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of the coding sequence. A lacZ gene with a 5' loxP site was inserted downstream of the coding sequence. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted downstream of the second loxP site. Western blot analysis confirmed reduced protein expression in the brain and heart. This allele is hypomorphic. Reporter expression requires cre-mediated removal of the coding sequence. (J:201812)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ntf3 Mutation: 24 strains or lines available

References

• Original: J:201812 Fox EA, et al., Loss of neurotrophin-3 from smooth muscle disrupts vagal gastrointestinal afferent signaling and satiation. Am J Physiol Regul Integr Comp Physiol. 2013 Dec;305(11):R1307-22
• All: 1 reference(s)