Hprt1^{tm1(tetO-EGFP/Rac1*)Shaw}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(tetO-EGFP/Rac1*)Shaw}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Andrey S Shaw
• MGI ID: MGI:5559077
• Synonyms: EGFP_CA-Rac1
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(tetO-EGFP/Rac1*)Shaw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206088
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Constitutively active, Inducible, Inserted expressed sequence, Null/knockout, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Hprt1^{tm1(tetO-EGFP/Rac1*)Shaw} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Rac1 (MGI:97845)

• Mutation details: The targeting construct introduced into the locus a tetracycline responsive promoter element (tetO), EGFP fused to the Rac1 sequence with a constitutively activating mutation that results in the amino acid substitution of lysine for glutamine at position 61 (Q61L) and a bovine polyadenylation sequence. (J:206088)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1273 strains or lines available

References

• Original: J:206088 Yu H, et al., Rac1 activation in podocytes induces rapid foot process effacement and proteinuria. Mol Cell Biol. 2013 Dec;33(23):4755-64
• All: 2 reference(s)