Igs7^{tm78.1(tetO-VSFPB1.2)Hze}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm78.1(tetO-VSFPB1.2)Hze}
• Name: intergenic site 7; targeted mutation 78.1, Hongkui Zeng
• MGI ID: MGI:5558051
• Synonyms: Ai78D, Ai78Deltahygro, Ai78(TITL-VSFPB)
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm78.1(tetO-VSFPB1.2)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The targeting vector was designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a modified Tet response element (TRE or tetO), a floxed-STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), the voltage-sensitive fluorescent protein Butterfly 1.2 coding sequence (details below), a WPRE sequence (to enhance mRNA stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, an AttB site, a PGK-5 hygro cassette, an RNA spice donor and a FRT5 site. Embryonic stem cells, previously targeted with FRT5-AttB-Hygro2 cassette-AttP-SV40pA-splice acceptor-PGKpA-FRT3 in the same locus, were re-transfected with the targeting vector and Flp recombinase vector. Mutant mice were bred with Gt(ROSA)26Sor^{tm3(phiC31*)Sor} mice to remove to remove the AttB/AttP-flanked PGK-hygro-SV40pA cassette and replace it with the recombined AttB/AttP site (AttL). The Ai78D allele is therefore TIGRE-frt3-Ins-TRE-LSL-VSFPB1.2-WPRE-bGHpA-Ins-AttL. (J:219930)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 64 strains or lines available

References

• Original: J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58
• All: 4 reference(s)