Adig^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Adig^{tm1.1(KOMP)Vlcg}
• Name: adipogenin; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5548852
• Synonyms: Adig^-
• Gene: Adig Location: Chr2:158344532-158350118 bp, + strand Genetic Position: Chr2, 78.72 cM
• Alliance: Adig^{tm1.1(KOMP)Vlcg} page
• IMPC: Adig gene page

Mutation origin

• Mutant Cell Line: 10117A-B5
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: Cre-mediated excision of the parental Adig^{tm1(KOMP)Vlcg} allele resulted in the removal of the neomycin selection cassette and critical exon(s) leaving behind the inserted lacZ reporter sequence. RT-PCR analysis revealed absence of mRNA expression in inguinal and epididymal white adipose tissue (WAT) and brown adipose tissue (BAT) as well as in liver and skeletal muscle tissue of homozygous mutant mice, thus confirming deletion of parts of exons 1 and 2. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:204739, J:304292)

Expression

In Mice Carrying this Mutation:	11 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Adig Mutation: 12 strains or lines available

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 4 reference(s)