Vamp2^tm1.1Jret
Targeted Allele Detail

Summary

• Symbol: Vamp2^tm1.1Jret
• Name: vesicle-associated membrane protein 2; targeted mutation 1.1, Jens Rettig
• MGI ID: MGI:5548222
• Synonyms: Syb2-mRFP
• Gene: Vamp2 Location: Chr11:68979354-68983207 bp, + strand Genetic Position: Chr11, 42.36 cM
• Alliance: Vamp2^tm1.1Jret page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205753
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A linker (GGSGGSGGT), RFP and self-excising neomycin resistance cassette was inserted at the 3' end of exon 5, replacing the stop codon. The final allele lacks the selection cassette. Western blot analysis confirmed normal protein expression. (J:205753)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vamp2 Mutation: 18 strains or lines available

References

• Original: J:205753 Matti U, et al., Synaptobrevin2 is the v-SNARE required for cytotoxic T-lymphocyte lytic granule fusion. Nat Commun. 2013;4:1439
• All: 5 reference(s)