Tg(tetO-AML1/ETO)8Dzh
Transgene Detail

Summary

• Symbol: Tg(tetO-AML1/ETO)8Dzh
• Name: transgene insertion 8, Dong-Er Zhang
• MGI ID: MGI:5547825
• Synonyms: AML1-ETO, TgPtet-AML1-ETO
• Transgene: Tg(tetO-AML1/ETO)8Dzh Location: unknown
• Alliance: Tg(tetO-AML1/ETO)8Dzh page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The transgenic construct contains a human AML1-ETO fusion protein sequence (coding for the translocation t(8;21) associated with acute myeloid leukemia (AML)) under the transcriptional control of the tetracycline transactivator responsive promoter (tetO). Inducible expression of AML1-ETO is observed in Northern and Western blot analysis. (J:64482)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Founder lines 2, 6, 7, and 13 were also established. When mice are crossed with the mice carrying the Tg(MMTVtTA)1Mam transgene, Founder lines 6, 8, and 13 express AML1-ETO in the bone marrow. These three lines have a similar pattern of expression. Founder line 7 expresses in bone marrow and peritoneal macrophages. No inducible expression is found in line 2.

References

• Original: J:64482 Rhoades KL, et al., Analysis of the role of AML1-ETO in leukemogenesis, using an inducible transgenic mouse model. Blood. 2000 Sep 15;96(6):2108-15
• All: 2 reference(s)