Prdm4^{tm1b(EUCOMM)Liz}
Targeted Allele Detail

Summary

• Symbol: Prdm4^{tm1b(EUCOMM)Liz}
• Name: PR domain containing 4; targeted mutation 1b, Elizabeth K Bikoff
• MGI ID: MGI:5547613
• Synonyms: Prdm4^LacZ
• Gene: Prdm4 Location: Chr10:85727828-85752958 bp, - strand Genetic Position: Chr10, 41.75 cM
• Alliance: Prdm4^{tm1b(EUCOMM)Liz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205013
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The EUCOMM targeting vector (project ID 45696) introduced an FRT site, lacZ gene, loxP site, neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site upstream of exon 6. A third loxP site was inserted downstream of exon 7. Exons 6 and 7 correspond to the PR/SET domain. Cre-mediated recombination removed the neomycin resistance cassette and exons 6 and 7, leaving the lacZ reporter intact. (J:205013)

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prdm4 Mutation: 41 strains or lines available

References

• Original: J:205013 Bogani D, et al., The PR/SET domain zinc finger protein Prdm4 regulates gene expression in embryonic stem cells but plays a nonessential role in the developing mouse embryo. Mol Cell Biol. 2013 Oct;33(19):3936-50
• All: 1 reference(s)