Exosc10^tm1.1Vlcg
Targeted Allele Detail

Summary

• Symbol: Exosc10^tm1.1Vlcg
• Name: exosome component 10; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5544774
• Gene: Exosc10 Location: Chr4:148642886-148666858 bp, + strand Genetic Position: Chr4, 78.76 cM
• Alliance: Exosc10^tm1.1Vlcg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:200671
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The following elements comprise the conditional by inversion (COIN) intron that was inserted into exon 2 (all splice sites are from rabbit HBB2 intron 2): between splice donor and acceptor sites, in reverse orientation, FRT site, hygromycin or neomycin drug selection cassette, FRT site, lox66 site, splice acceptor site, EGFP cDNA, rabbit HBB2 3' UTR (for poly(A) signal), and lox71 site. This splits exon 2 into two parts, 412 and 1,018 bp in length. Flp-mediated recombination removed the drug selection cassette. The allele expresses a wildtype transcript because the inserted COIN cassette is spliced out. Because the two lox sites are in reverse complement orientation and create a lox72 and loxP site upon recombination, Cre-mediated recombination will irreversibly invert the inserted sequence, creating a reporter knockout allele. (J:200671)

Expression

In Mice Carrying this Mutation:

48 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Exosc10 Mutation: 61 strains or lines available

References

• Original: J:200671 Economides AN, et al., Conditionals by inversion provide a universal method for the generation of conditional alleles. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88
• All: 3 reference(s)