Knstrn^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Knstrn^{tm1d(KOMP)Wtsi}
• Name: kinetochore-localized astrin/SPAG5 binding; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5544487
• Synonyms: D2Ertd750e
• Gene: Knstrn Location: Chr2:118644470-118667691 bp, + strand Genetic Position: Chr2, 59.46 cM
• Alliance: Knstrn^{tm1d(KOMP)Wtsi} page
• IMPC: Knstrn gene page

Mutation origin

• Mutant Cell Lines: EPD0125_3_E05, EPD0125_3_H05
• Germline Transmission: Earliest citation of germline transmission: J:232595
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 118647900 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The original cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 118648692. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" allele was created by flp recombinase expression in mice carrying this allele and subsequent CMV-cre expression resulted in a knockout mouse lacking exon 3. Western blot analysis of adult testis extracts confirmed the absence of protein in homozygous mutant mice. (J:232595)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Knstrn Mutation: 23 strains or lines available

Notes

Cell line EPD0125_3_E05 was successfully used to make chimeric mice. Germline transmission was accomplished. J:232595

References

• Original: J:232595 Grey C, et al., SKAP, an outer kinetochore protein, is required for mouse germ cell development. Reproduction. 2016 Mar;151(3):239-51
• All: 1 reference(s)