Sox17tm4.1(icre/ERT2)Heli
Targeted Allele Detail
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Symbol: |
Sox17tm4.1(icre/ERT2)Heli |
Name: |
SRY (sex determining region Y)-box 17; targeted mutation 4.1, Heiko Lickert |
MGI ID: |
MGI:5543602 |
Synonyms: |
Sox17CreERT2, Sox17CreERT2deltaneo |
Gene: |
Sox17 Location: Chr1:4561154-4567577 bp, - strand Genetic Position: Chr1, 1.65 cM
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Alliance: |
Sox17tm4.1(icre/ERT2)Heli page
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Allele Type: |
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Targeted (Inducible, Recombinase) |
Inducer: |
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tamoxifen |
Mutation: |
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Insertion
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Sox17tm4.1(icre/ERT2)Heli expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Sox17 (MGI:107543) |
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Mutation details: A targeting vector was designed to delete the translational stop codon of Sox17 and to introduce the codon-optimized cre fused to the triple-mutant (G400V, M543A and L544A) human estrogen receptor ligand binding domain (ERT2) sequence preceded by a viral 2A peptide sequence into exon 5. Homologous recombination in ES cells integrated the cre/ERT2 cassette and Frt-flanked neomycin marker so that the targeted Sox17 allele will produce an bicistronic mRNA that will be co-translationally cleaved into equimolar amounts of Sox17 and cre. Chimeras were crossed to a Flp deleter line to remove the neo selection marker.
(J:204611)
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Generation of Sox17tm4.1(icre/ERT2)Heli. |
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Sox17
(mouse)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Sox17 Mutation: |
28 strains or lines available
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Original: |
J:204611 Engert S, et al., The Sox17CreERT2 knock-in mouse line displays spatiotemporal activation of Cre recombinase in distinct Sox17 lineage progenitors. Genesis. 2013 Nov;51(11):793-802 |
All: |
1 reference(s) |
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