Mlkl^em1Jhan
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mlkl^em1Jhan
• Name: mixed lineage kinase domain-like; endonuclease-mediated mutation 1, Jiahuai Han
• MGI ID: MGI:5538612
• Gene: Mlkl Location: Chr8:112038429-112064809 bp, - strand Genetic Position: Chr8, 57.98 cM, cytoband D3
• Alliance: Mlkl^em1Jhan page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: TALENs targeting exon 1 induced a 2 bp deletion at the 52nd base of the coding region. This deletion results in a stop codon and premature truncation after glutamine 17 (p.C18*fs*1). Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts, bone marrow-derived macrophages (BMDMs), peritoneal macrophages, thymus, muscle, colon, intestine, heart, liver, spleen, lung, kidney and brain. (J:204622)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mlkl Mutation: 32 strains or lines available

References

• Original: J:204622 Wu J, et al., Mlkl knockout mice demonstrate the indispensable role of Mlkl in necroptosis. Cell Res. 2013 Aug;23(8):994-1006
• All: 11 reference(s)