Jak2^tm2.2Jlvl
Targeted Allele Detail

Summary

• Symbol: Jak2^tm2.2Jlvl
• Name: Janus kinase 2; targeted mutation 2.2, Jean-Luc Villeval
• MGI ID: MGI:5538399
• Synonyms: JAK2^V617F, JAK2^VF
• Gene: Jak2 Location: Chr19:29229228-29290480 bp, + strand Genetic Position: Chr19, 23.73 cM
• Alliance: Jak2^tm2.2Jlvl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:202234
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: A loxP site was inserted upstream of exon 13. A lox511 site, a inverted and modified exon 13 with a point mutation (GTC to TTC) that results in the amino acid substitution of phenylalanine for valine at position 617 (V617F) and a loxP site were inserted downstream of of exon 13. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted downstream of the modified exon 13 and left a lox511 site downstream of the modified exon 13. Cre-mediated recombination inverted the modified and endogenous exon 13 to allow for expression of the modified exon 13. (J:202234)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Jak2 Mutation: 57 strains or lines available

References

• Original: J:202234 Hasan S, et al., JAK2V617F expression in mice amplifies early hematopoietic cells and gives them a competitive advantage that is hampered by IFNalpha. Blood. 2013 Aug 22;122(8):1464-77
• All: 8 reference(s)