Gzmb^tm1Asmv
Targeted Allele Detail

Summary

• Symbol: Gzmb^tm1Asmv
• Name: granzyme B; targeted mutation 1, Anne-Marie Schmitt-Verhulst
• MGI ID: MGI:5532521
• Synonyms: Gzmb-tdTomato delta neo, Gzmb^tm1.1Clbr, Gzmb-Tom-KI
• Gene: Gzmb Location: Chr14:56496295-56499717 bp, - strand Genetic Position: Chr14, 28.19 cM, cytoband D3
• Alliance: Gzmb^tm1Asmv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:203720
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Mutation details: Insertion of the tdTomato cDNA plus a 12 amino acid linker and a self-excising neomycin resistance cassette replaced the stop codon. A neomycin resistance cassette was deleted in ES cells prior to the creation of mice. (J:203720)

Generation of the Gzmb^tm1Asmv allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gzmb Mutation: 34 strains or lines available

References

• Original: J:203720 Mouchacca P, et al., Visualization of cytolytic T cell differentiation and granule exocytosis with T cells from mice expressing active fluorescent granzyme B. PLoS One. 2013;8(6):e67239
• All: 7 reference(s)