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Tg(Pdpn-icre)14Biat
Transgene Detail
Summary
Symbol: Tg(Pdpn-icre)14Biat
Name: transgene insertion 14, Biomodels Austria
MGI ID: MGI:5526988
Synonyms: Pdpn-Cre, transgene insertion 14, Thomas Ruelicke
Transgene: Tg(Pdpn-icre)14Biat  Location: unknown  
Alliance: Tg(Pdpn-icre)14Biat page
Transgene
origin
Strain of Origin:  C57BL/6N
Transgene
description
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
 
Tg(Pdpn-icre)14Biat expression driven by 1 gene
 
Mutation detailsA BAC containing the podoplanin locus was modified, replacing exon 1 with a codon-optimized cre sequence and polyadenylation site. The cre sequence was integrated downstream of the start codon. Microinjection of the construct into pronuclei of zygotes produced 17 founders with 3 (406-408) positive for cre recombinase and the 5' ATG region by southern blot. These founders were designated 6, 12, and 14. When crossed to EYFP reporter mice, line 12 offspring did not have detectable cre expression, while line 6 offspring had some EYFP expression in the CD45+ve non-stromal cell compartment. Thus line 6 was excluded from further analysis. Offspring from line 14 had no cre activity in non-stromal cells and substantial activity in CD45-ve LN (lymph node) cells (in 15% in LEC and FRC fractions and very low expression in BECs or absent in the double-negative fractions.) (J:203412)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Pdpn (species not specified)
Summary of all recombinase alleles driven by Pdpn.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:203412 Onder L, et al., A novel bacterial artificial chromosome-transgenic podoplanin-cre mouse targets lymphoid organ stromal cells in vivo. Front Immunol. 2011;2:50
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
07/05/2024
MGI 6.24
The Jackson Laboratory