Tg(Thy1-GCaMP6s)GP4.3Dkim
Transgene Detail

Summary

• Symbol: Tg(Thy1-GCaMP6s)GP4.3Dkim
• Name: transgene insertion GP4.3, Douglas Kim
• MGI ID: MGI:5523977
• Synonyms: GP4.3
• Transgene: Tg(Thy1-GCaMP6s)GP4.3Dkim Location: unknown
• Alliance: Tg(Thy1-GCaMP6s)GP4.3Dkim page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The mouse promoter drives neuron-specific expression of this transgene that includes a mutagenized green fluorescent calcium indicator (GCaMP6s), woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation sequence. GCaMP consists of a circularly permutated green fluorescent protein (cpGFP), the calcium-binding protein calmodulin (CaM), and CaM-interacting M13 peptide. This GCaMP6s variant of GCaMP incorporates a K78H mutation in cpEGFP, as well as T381R, S383T, and R392G mutations in CaM. Line GP4.3 was generated. (J:101977, J:203036)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 113 reference(s)