Tdrkh^tm1.2Hfl
Targeted Allele Detail

Summary

• Symbol: Tdrkh^tm1.2Hfl
• Name: tudor and KH domain containing protein; targeted mutation 1.2, Haifan Lin
• MGI ID: MGI:5521576
• Gene: Tdrkh Location: Chr3:94320580-94341975 bp, + strand Genetic Position: Chr3, 40.62 cM, cytoband F2
• Alliance: Tdrkh^tm1.2Hfl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:197673
• Parent Cell Line: D2 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neomycin resistance cassette and left exons 2 through 4 floxed. Cre-mediated recombination removed exons 2 through 4. Western blot analysis confirmed the absence of protein expression in testis. (J:197673)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tdrkh Mutation: 22 strains or lines available

References

• Original: J:197673 Saxe JP, et al., Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline. EMBO J. 2013 Jul 3;32(13):1869-85
• All: 2 reference(s)