H2-Ab1^b-tm1.1Jssh
Targeted Allele Detail

Summary

• Symbol: H2-Ab1^b-tm1.1Jssh
• Name: histocompatibility 2, class II antigen A, beta 1; targeted mutation 1.1, Jeoung-Sook Shin
• MGI ID: MGI:5521551
• Synonyms: H2-Ab1^tm1.1Jssh
• Gene: H2-Ab1 Location: Chr17:34482201-34488392 bp, + strand Genetic Position: Chr17, 17.98 cM
• Alliance: H2-Ab1^b-tm1.1Jssh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201198
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

Allele Type: Targeted (Not Specified) Mutations: Insertion, Nucleotide substitutions Mutation details: A floxed neomycin resistance cassette was inserted upstream of a modified exon 4 in which nucleotide substitutions (CACAGGAGTCAGAAA to CACAGATCTCAAAGG) result in the amino acid substitution of arginine for lysine at position 253 (K253R). This mutation prevents ubiquitination. Cre-mediated recombination removed the neomycin resistance cassette. (J:201198)

Generation of Marchf1^tm1.1Sish/Marchf1^tm1.1Sish mice

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H2-Ab1 Mutation: 81 strains or lines available

References

• Original: J:201198 Oh J, et al., MARCH1-mediated MHCII ubiquitination promotes dendritic cell selection of natural regulatory T cells. J Exp Med. 2013 Jun 3;210(6):1069-77
• All: 2 reference(s)