Piwil2^tm2.2Doca
Targeted Allele Detail

Summary

• Symbol: Piwil2^tm2.2Doca
• Name: piwi-like RNA-mediated gene silencing 2; targeted mutation 2.2, Donal O'Carroll
• MGI ID: MGI:5521293
• Synonyms: MiliNull
• Gene: Piwil2 Location: Chr14:70609926-70666832 bp, - strand Genetic Position: Chr14, 36.32 cM
• Alliance: Piwil2^tm2.2Doca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199259
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 21. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 22. Flp-mediated recombination removed the neomycin resistance cassette and left exons 21 and 22 floxed. Cre-mediated recombination removed exons 21 and 22. Western blot analysis confirmed the absence of protein expression in the testis. (J:199259)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Piwil2 Mutation: 54 strains or lines available

References

• Original: J:199259 Di Giacomo M, et al., Multiple epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis. Mol Cell. 2013 May 23;50(4):601-8
• All: 5 reference(s)