Tnc^{tm1(cre/ERT2)Cmha}
Targeted Allele Detail

Summary

• Symbol: Tnc^{tm1(cre/ERT2)Cmha}
• Name: tenascin C; targeted mutation 1, Chuan-Ming Hao
• MGI ID: MGI:5520052
• Synonyms: Tenascin-C-CreER2, Tnc^YFP-CreER
• Gene: Tnc Location: Chr4:63878022-63965252 bp, - strand Genetic Position: Chr4, 34.06 cM
• Alliance: Tnc^{tm1(cre/ERT2)Cmha} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:202522
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tnc^{tm1(cre/ERT2)Cmha} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tnc (MGI:101922)

• Mutation details: Using homologous recombination, a cre/ERT2-IRES-EGFP cassette was inserted immediately upstream of the translation start ATG codon in exon 2 of the tenascin C gene. A neo selection marker flanked by frt sites followed the cre cassette. EGFP expression is similar to endogenous Tnc expression in the kidney inner medulla interstitial cells. No significant cre-dependent reporter activity is observed in absence of tamoxifen treatment. (J:202522)

Generation of Tnc^{tm1(cre/ERT2)Cmha}.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tnc (mouse) Summary of all recombinase alleles driven by Tnc. Comparative matrix view of recombinase activities Alliance mouse Tnc gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnc Mutation: 130 strains or lines available

References

• Original: J:202522 He W, et al., Generation of a Tenascin-C-CreER2 Knockin Mouse Line for Conditional DNA Recombination in Renal Medullary Interstitial Cells. PLoS One. 2013;8(11):e79839
• All: 7 reference(s)