Gt(ROSA)26Sor^{tm3(Kit/KIT*)Hsc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(Kit/KIT*)Hsc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Hubert Schorle
• MGI ID: MGI:5516508
• Synonyms: GFP-2A-KITD816V, Gt(ROSA)26sor^{Tm1(GFP-cKIT*)Hsc}, R26-LSL-KIT^D816V
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(Kit/KIT*)Hsc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:214144
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Gt(ROSA)26Sor^{tm3(Kit/KIT*)Hsc} expresses 2 genes
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  mouse

  Kit (MGI:96677)

  This allele expresses a fusion of the mouse and human genes

  human

  KIT (3815)

  Kit	(MGI:96677)

• Mutation details: A floxed stop cassette and a GFP gene linked by a viral 2A sequence to a cDNA consisting of the mouse Kit extracellular and transmembrane domains and the human KIT intracellular domain with a D816V substitution was inserted in intron 1 via homologous recombination. (J:214144)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:214144 Haas N, et al., Kit transduced signals counteract erythroid maturation by MAPK-dependent modulation of erythropoietin signaling and apoptosis induction in mouse fetal liver. Cell Death Differ. 2015 May;22(5):790-800
• All: 1 reference(s)