Akap9^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Akap9^{tm1b(KOMP)Wtsi}
• Name: A kinase anchor protein 9; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5516002
• Gene: Akap9 Location: Chr5:3977410-4130204 bp, + strand Genetic Position: Chr5, 2.26 cM, cytoband A2
• Alliance: Akap9^{tm1b(KOMP)Wtsi} page
• IMPC: Akap9 gene page

Mutation origin

• Mutant Cell Line: EPD0033_5_D02
• Germline Transmission: Earliest citation of germline transmission: J:198499
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 4018123 of Chromosome 5 upstream of the critical exon 8 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 8 at position 4019078. The critical exon 8 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Cre-mediated recombination removed exon 8 and left the reporter construct intact. Western blot analysis confirmed the absence of protein expression in the pancreas and testis. (J:198499)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Akap9 Mutation: 180 strains or lines available

Notes

Cell line EPD0033_5_D02 was successfully used to make chimeric mice. Germline transmission was accomplished. J:198499

References

• Original: J:198499 Schimenti KJ, et al., AKAP9 is essential for spermatogenesis and sertoli cell maturation in mice. Genetics. 2013 Jun;194(2):447-57
• All: 1 reference(s)