Hprt1^{tm2(CAG-mCherry/Villin*)Syr}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm2(CAG-mCherry/Villin*)Syr}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 2, Sylvie Robine
• MGI ID: MGI:5512852
• Synonyms: mCherry-Villin deltaSev
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm2(CAG-mCherry/Villin*)Syr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196199
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Hprt1^{tm2(CAG-mCherry/Villin*)Syr} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  VIL1 (7429)

  Vil1	(MGI:98930)

• Mutation details: A CAG promoter was used to drive expression of a floxed chloramphenicol acetyltransferase. An mCherry tagged Villin sequencethat has been modified to eliminate its F-actin severing activity was inserted further downstream so that it only becomes active after cre excision of chloramphenicol acetyltransferase. (J:196199)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:196199 Ubelmann F, et al., Enterocyte loss of polarity and gut wound healing rely upon the F-actin-severing function of villin. Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):E1380-9
• All: 1 reference(s)