Gad2^tm1.1Ksvo
Targeted Allele Detail

Summary

• Symbol: Gad2^tm1.1Ksvo
• Name: glutamic acid decarboxylase 2; targeted mutation 1.1, Karel Svoboda
• MGI ID: MGI:5512722
• Synonyms: Gad2^2A-mCherry, Gad2-2A-nls-mcherry, Gad2-T2A-NLS-mCherry
• Gene: Gad2 Location: Chr2:22512262-22583889 bp, + strand Genetic Position: Chr2, 15.15 cM, cytoband A-B
• Alliance: Gad2^tm1.1Ksvo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), and a nuclear localized mCherry in in-frame after the last codon of the Gad2 gene. A frt-flanked neo was places downstream of the mCherry. Flp-mediated recombination removed the neomycin resistance cassette. (J:221246)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gad2 Mutation: 60 strains or lines available

References

• Original: J:221246 Peron SP, et al., A Cellular Resolution Map of Barrel Cortex Activity during Tactile Behavior. Neuron. 2015 May 6;86(3):783-99
• All: 25 reference(s)