Fzd3^tm2.1Nat
Targeted Allele Detail

Summary

• Symbol: Fzd3^tm2.1Nat
• Name: frizzled class receptor 3; targeted mutation 2.1, Jeremy Nathans
• MGI ID: MGI:5510964
• Synonyms: Fz3 CKO
• Gene: Fzd3 Location: Chr14:65429898-65499912 bp, - strand Genetic Position: Chr14, 34.09 cM
• Alliance: Fzd3^tm2.1Nat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The following elements were inserted into the mouse Fzd3 (frizzled homolog 3 (Drosophila)) locus via homologous recombination: a loxP site in intron 2, a 3HA (hemagglutinin) epitope in the coding region between the cysteine-rich domain and the first transmembrane domain, a loxP site in intron 3, and an Frt-flanked PGK-neo cassette. The neomycin cassette was removed by germline Flp-mediated recombination. (J:101977, J:207894)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fzd3 Mutation: 32 strains or lines available

References

• Original: J:207894 Hua ZL, et al., Frizzled3 controls axonal development in distinct populations of cranial and spinal motor neurons. Elife. 2013;2:e01482
• All: 5 reference(s)