Setd5^tm1.1Mgn
Targeted Allele Detail

Summary

• Symbol: Setd5^tm1.1Mgn
• Name: SET domain containing 5; targeted mutation 1.1, Mark Magnuson
• MGI ID: MGI:5509467
• Synonyms: Setd5^GFP
• Gene: Setd5 Location: Chr6:113054326-113130393 bp, + strand Genetic Position: Chr6, 52.73 cM, cytoband E3
• Alliance: Setd5^tm1.1Mgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237500
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The pRosa26.Ex1.Setd5GFP exchange vector was made on a backbone of a basal Rosa26 exchange vector which contains a 5.166 kb sequence from the Rosa26 locus, Lox71/Lox2272 sites, and flrted (flanked by FRT) Pgk-Hygro selection cassette that is used for positive selection after RMCE. A GFP-SV40 polyA sequence was inserted into exon 1 of the Setd5 gene which is located upstream of the Rosa26 exon 1 and is transcribed in the opposite orientation. Gene targeting and RMCE was performed in mESCs derived from 129S6 mice. The chimeric mice were crossed with Flpe mice to remove the hygromycin resistance gene. (J:237500)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Setd5 Mutation: 120 strains or lines available

References

• Original: J:237500 Osipovich AB, et al., Setd5 is essential for mammalian development and the co-transcriptional regulation of histone acetylation. Development. 2016 Dec 15;143(24):4595-4607
• All: 3 reference(s)