Pou5f1^tm1Grc
Targeted Allele Detail

Summary

• Symbol: Pou5f1^tm1Grc
• Name: POU domain, class 5, transcription factor 1; targeted mutation 1, Gerald R Crabtree
• MGI ID: MGI:5504452
• Gene: Pou5f1 Location: Chr17:35816929-35821674 bp, + strand Genetic Position: Chr17, 18.69 cM
• Alliance: Pou5f1^tm1Grc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199710
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A BAC containing the mouse locus was manipulated through recombineering. The construct was flanked with homology arms of approximately 3.7 kbp upstream and 10.9 kbp downstream. Two distinct DNA binding sequence arrays were added at -277 bp upstream of the transcriptional start of of the gene, 12 x ZFHD1 (TAATGATGGGCG) and 5 x Gal4 (CGGAGTACTGTCCTCCGAG). A nuclear EGFP was inserted at the ATG of exon 1, exons 2-5 were deleted after insertion to avoid expression of endogenous Pou5f1 from the reporter allele. A floxed neomycin resistance cassette was inserted behind the EGFP reporter for positive selection and thymidine kinase was inserted behind the homology arms for negative selection. (J:199709, J:199710)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pou5f1 Mutation: 82 strains or lines available

References

• Original: J:199710 Crabtree GR, Direct Data Submission for Pou5fl^tm1Grc. MGI Direct Data Submission. 2013
• All: 2 reference(s)