Gt(Ayu21-B137)Imeg
Gene trapped Allele Detail

Summary

• Symbol: Gt(Ayu21-B137)Imeg
• Name: gene trap Ayu21-B137, Institute of Molecular Embryology and Genetics
• MGI ID: MGI:5503017
• Gene: Gt(Ayu21-B137)Imeg Location: unknown Genetic Position: Chr4, Syntenic
• Alliance: Gt(Ayu21-B137)Imeg page

Mutation origin

• Mutant Cell Line: Ayu21-B137
• Germline Transmission: Earliest citation of germline transmission: J:195133
• Parent Cell Line: KTPU8 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Gene trapped (Not Specified)
• Mutation: Insertion of gene trap vector
• Mutation details: The "promoter trap" gene trap vector pU21 contains 1.2 kb of intron 1 and part of exon 2, including the splice acceptor, from the mouse engrailed 2 gene (En2) followed by the Betageo beta-galactosidase/neomycin resistance fusion gene with the SV40 and mouse Pgk1 polyadenylation signals. Three stop codons occur in the exon sequence upstream of and in frame with the Betageo translation initiation codon. loxP and FRT sites used for 5' RACE analysis and for in vitro exchange of other sequences for Betageo are present. (J:195133)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(Ayu21-B137)Imeg Mutation: 1 strain or line available

References

• Original: J:195133 Semba K, et al., Ectopic expression of Ptf1a induces spinal defects, urogenital defects, and anorectal malformations in Danforth's short tail mice. PLoS Genet. 2013 Feb;9(2):e1003204
• All: 2 reference(s)