Ldb1^tm1.1Gsv
Targeted Allele Detail

Summary

• Symbol: Ldb1^tm1.1Gsv
• Name: LIM domain binding 1; targeted mutation 1.1, Frank Grosveld
• MGI ID: MGI:5501525
• Gene: Ldb1 Location: Chr19:46020009-46033653 bp, - strand Genetic Position: Chr19, 38.75 cM
• Alliance: Ldb1^tm1.1Gsv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196469
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 1. A floxed neomycin (neo) resistance cassette with a mitochondrial localization signal (MLS)-CFP was inserted downstream of exon 2. Cre-mediated recombination removed the neo cassette and the first two coding exon. Western blot analysis confirmed the absence of protein expression in embryos. (J:196469)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ldb1 Mutation: 35 strains or lines available

References

• Original: J:196469 Mylona A, et al., Genome-wide analysis shows that Ldb1 controls essential hematopoietic genes/pathways in mouse early development and reveals novel players in hematopoiesis. Blood. 2013 Apr 11;121(15):2902-13
• All: 1 reference(s)