Dbn1^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dbn1^{tm1b(KOMP)Wtsi}
• Name: drebrin 1; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5501061
• Gene: Dbn1 Location: Chr13:55621241-55635874 bp, - strand Genetic Position: Chr13, 30.06 cM
• Alliance: Dbn1^{tm1b(KOMP)Wtsi} page
• IMPC: Dbn1 gene page

Mutation origin

• Mutant Cell Line: EPD0211_3_A05
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 55628889 of Chromosome 13 upstream of exon 1 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene cassette under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 55631060. The critical exons are thus flanked by loxP sites. This reporter knockout allele was created through subsequent cre-mediated recombination, deleting exons 1-6 and keeping the lacZ and neo cassettes in place. (J:148605, J:173534, J:188991)

Expression

In Mice Carrying this Mutation:	619 assay results
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Dbn1 Mutation: 44 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 13 reference(s)