Eomes^{tm1.1(cre/ERT2)Sjar}
Targeted Allele Detail

Summary

• Symbol: Eomes^{tm1.1(cre/ERT2)Sjar}
• Name: eomesodermin; targeted mutation 1.1, Sebastian J Arnold
• MGI ID: MGI:5499762
• Synonyms: Eomes^CreER
• Gene: Eomes Location: Chr9:118307259-118315176 bp, + strand Genetic Position: Chr9, 70.21 cM, cytoband F3
• Alliance: Eomes^{tm1.1(cre/ERT2)Sjar} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198846
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Eomes^{tm1.1(cre/ERT2)Sjar} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Eomes (MGI:1201683)

• Mutation details: A cre/ERT2-polyA cassette and loxP-flanked neomycin selection cassette were inserted into the Eomes locus at the translational start site in exon 1 by homologous recombination. This resulted in deletion of about 500 bp of the coding region of exon 1 (creating a null allele). The neo selection cassette was deleted in the final knockin allele. In situ hybridization demonstrated that embryonic expression of cre/ERT2 recapitulated the spatiotemporal expression pattern of endogenous Eomes. (J:198846)

Generation of Eomes^{tm1.1(cre/ERT2)Sjar}.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Eomes (mouse) Summary of all recombinase alleles driven by Eomes. Alliance mouse Eomes gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eomes Mutation: 42 strains or lines available

References

• Original: J:198846 Pimeisl IM, et al., Generation and characterization of a tamoxifen-inducible Eomes(CreER) mouse line. Genesis. 2013 Oct;51(10):725-33
• All: 9 reference(s)