Asphd1^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Asphd1^{tm1a(EUCOMM)Hmgu}
• Name: aspartate beta-hydroxylase domain containing 1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5497168
• Gene: Asphd1 Location: Chr7:126545159-126548754 bp, - strand Genetic Position: Chr7, 69.25 cM
• Alliance: Asphd1^{tm1a(EUCOMM)Hmgu} page
• IMPC: Asphd1 gene page

Mutation origin

• Mutant Cell Lines: HEPD0862_1_A02, HEPD0862_1_A03, HEPD0862_1_B01, HEPD0862_1_D01, HEPD0862_1_E02, HEPD0862_1_H02, HEPD0862_1_H03
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_GT1_LF2A_LacZ_BetactP_neo
• Mutation details: The L1L2_GT1_LF2A_LacZ_BetactP_neo cassette was inserted at position 126544924 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 126546450. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Asphd1 Mutation: 19 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)