Ins2^tm1Rll
Targeted Allele Detail

Summary

• Symbol: Ins2^tm1Rll
• Name: insulin II; targeted mutation 1, Rudolph L Leibel
• MGI ID: MGI:5494871
• Synonyms: Ins2-Gfp
• Gene: Ins2 Location: Chr7:142232393-142233463 bp, - strand Genetic Position: Chr7, 88.0 cM
• Alliance: Ins2^tm1Rll page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196410
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of the gene near the translation initiation site was replaced with an EGFP gene and kanamycin resistance cassette. Western blot analysis confirmed the expression of a 27 kDa band not present in wild-type samples. Immunohistochemistry confirmed expression in 96% of insulin-positive beta cells but not in the brain, kidneys or liver. (J:196410)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ins2 Mutation: 90 strains or lines available

References

• Original: J:196410 Wakae-Takada N, et al., Molecular basis for the regulation of islet beta cell mass in mice: the role of E-cadherin. Diabetologia. 2013 Apr;56(4):856-66
• All: 2 reference(s)