Foxa2^{tm1(cre/ERT2)Hssk}
Targeted Allele Detail

Summary

• Symbol: Foxa2^{tm1(cre/ERT2)Hssk}
• Name: forkhead box A2; targeted mutation 1, Hiroshi Sasaki
• MGI ID: MGI:5490029
• Synonyms: Foxa2^{nEGFP-CreERT2}, Foxa2^{nEGFP-CreERT2/1}
• Gene: Foxa2 Location: Chr2:147884797-147888889 bp, - strand Genetic Position: Chr2, 73.38 cM
• Alliance: Foxa2^{tm1(cre/ERT2)Hssk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194054
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Foxa2^{tm1(cre/ERT2)Hssk} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Foxa2 (MGI:1347476)

• Mutation details: Recombineering of the BAC clone RP24-386G1 containing the Foxa2 locus generated a targeting vector designed to replace the stop codon of Foxa2 and introduce sequences encoding the ERAV 2A peptide, a human histone H2B/EGFP fusion protein (protein is expressed in the cell nucleus), the Thosea asigna virus 2A peptide and cre/ERT2 fusion protein. A self-excising ACN cassette (containing the neo resistance marker) was also included. The vector was electroporated into ES cells for homologous recombination. Correctly targeted clones were used to produce chimeras. Chimeras were bred to C57BL/6J females, with the ACN cassette self-excising during passage through the germline. Two independent ES cell clones (3 and 22) produced mouse lines which had the same phenotpe, and the line derived from #22 was analyzed further. (J:194054)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Foxa2 (mouse) Summary of all recombinase alleles driven by Foxa2. Comparative matrix view of recombinase activities Alliance mouse Foxa2 gene page

Expression

In Mice Carrying this Mutation:

12 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxa2 Mutation: 26 strains or lines available

References

• Original: J:194054 Imuta Y, et al., Generation of knock-in mice that express nuclear enhanced green fluorescent protein and tamoxifen-inducible Cre recombinase in the notochord from Foxa2 and T loci. Genesis. 2013 Mar;51(3):210-8
• All: 8 reference(s)