Tg(Prnp*P101L)174Sbp
Transgene Detail

Summary

• Symbol: Tg(Prnp*P101L)174Sbp
• Name: transgene insertion 174, Stanley B Prusiner
• MGI ID: MGI:5487544
• Synonyms: Tg(GSSPrP)174, Tg(MoPrP-P101L)174
• Transgene: Tg(Prnp*P101L)174Sbp Location: unknown
• Alliance: Tg(Prnp*P101L)174Sbp page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp*P101L)174Sbp expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Prnp (MGI:97769)

• Mutation details: This transgene consists of the murine Prnp with a proline to leucine substitution at codon 101 (equivalent to codon 102 in human PRNP). This construct was generated using a chimeric murine cosmid constructed by exchanging the BamHI-SalI fragment containing the open reading frame from a recombinant NZW genomic clone for the corresponding fragment in an ILn/J mouse cosmid. An approximate 100-nucleotide region flanking codon 101 leucine was modified to yield DNA sequence homology to hamster Prnp while retaining amino acid homology to mouse Prnp. Analysis indicated that this line carries approximately 64 haploid transgene copies inserted into a single autosomal site. Three lines were created: 174, 180, and 196. (J:127373)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:127373 Hsiao KK, et al., Spontaneous neurodegeneration in transgenic mice with mutant prion protein. Science. 1990 Dec 14;250(4987):1587-90
• All: 1 reference(s)