Zbtb7b^tm5.1Itan
Targeted Allele Detail

Summary

• Symbol: Zbtb7b^tm5.1Itan
• Name: zinc finger and BTB domain containing 7B; targeted mutation 5.1, Ichiro Taniuchi
• MGI ID: MGI:5486336
• Synonyms: Thpok^gfp;deltaTE
• Gene: Zbtb7b Location: Chr3:89284953-89300976 bp, - strand Genetic Position: Chr3, 39.09 cM, cytoband A1-A2
• Alliance: Zbtb7b^tm5.1Itan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193321
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (129 x C57BL/6)F1

Mutation description

• Allele Type: Targeted (Modified regulatory region, Reporter)
• Mutation: Intragenic deletion
• Mutation details: ES cells were used that had been previously targeted for the insertion of a GFP reporter. The targeting vector deleted the thymic enhancer (TE) in the distal regulatory element while leaving the silencer sequence intact. A floxed neomycin selection was also inserted adjacent to the silencer element. The resistance cassette was eventually excised by transient cre expression. (J:193321)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zbtb7b Mutation: 26 strains or lines available

Notes

Targeting was accomplished in ES cells in which this gene had already been modified by the insertion of a GFP reporter. Initial targeting was accomplished in M1/M1-1 ES cells derived from (129 x C57BL/6)F1 mice.

References

• Original: J:193321 Muroi S, et al., Cutting edge: fine-tuning of thpok gene activation by an enhancer in close proximity to its own silencer. J Immunol. 2013 Feb 15;190(4):1397-401
• All: 2 reference(s)