Rr26^tm1Svok
Targeted Allele Detail

Summary

• Symbol: Rr26^tm1Svok
• Name: regulatory region 26; targeted mutation 1, Steven Vokes
• MGI ID: MGI:5486166
• Synonyms: Gremlin^deltaGRE1
• Gene: Rr26 Location: Chr2:113471188-113471625 bp Genetic Position: Chr2, Syntenic
• Alliance: Rr26^tm1Svok page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207959
• Parent Cell Line: AV3 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A 7.5kb genomic fragment containing the Gremlin cis regulatory region was digested from BAC RP23-113H17 and a loxP site was inserted upstream and an FRT site flanked neomycin resistance gene cassette and a second loxP cassette downstream of the enhancer (chr2:113640843-113641280, Build 38). After homologous recombination, cre-mediated recombination removed the enhancer/silencer and selection cassette. The enhancer regulates Grem1 but is located in intron 13 of Fmn1. (J:207959)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr26 Mutation: 0 strains or lines available

References

• Original: J:207959 Li Q, et al., A Gli silencer is required for robust repression of gremlin in the vertebrate limb bud. Development. 2014 May;141(9):1906-14
• All: 1 reference(s)